|
KMID : 1094720080130050624
|
|
Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 5 p.624 ~ p.631
|
|
|
Proteome analysis on differentially expressed proteins of the fat body of two silkworm breeds, Bombyx mori , exposed to heat shock exposure
|
|
Moghaddam S. Hossein Hosseini
Du Xin
Li Jun
Cao Jin-Ru
Zhong Bo-Xiong
Chen Yu-Yin
|
|
|
Abstract
|
|
|
|
Proteomes of heat tolerant (multivoltine) and heat susceptible (bivoltine) silkworms (Bombyx mori) in response to heat shock were studied. Detected proteins from fat body were identified by using MALDI-TOF/TOF spectrometer, MS/MS, and MS analysis. Eight proteins, including small heat shock proteins (sHSPs) and HSP70, were expressed similarly in both breeds, while 4 protein spots were expressed specifically in the bivoltine breed and 12 protein spots were expressed specifically in the multivoltine breed. In the present proteomics approach, 5 separate spots of sHSP proteins (HSP19.9, HSP20.1, HSP20.4, HSP20.8, and HSP21.4) were identified. Protein spot intensity of sHSPs was lower in the multivoltine breed than in the bivoltine breed after the 45°C heat shock treatment, while the difference between two breeds was not significant after the 41°C heat shock treatment. These results indicated that some other mechanisms might be engaged in thermal tolerance of multivotine breed except for the expression of sHSP and HSP70. There were visible differences in the intensity of heat shock protein expression between male and female, however, differences were not statistically significant.
|
|
|
KEYWORD
|
|
|
proteome analysis, heat shock proteins, silkworm, 2D electrophoresis, mass spectrometry
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|